Atcc hl-60
Always get your cells from the source (ATCC for the HL-60xs or DSMZ for the PLB-985s). Using cells that "someone got from someone" (as we too often see, though this may not be your case) is bound
.. Article Title: A Glycovariant of Human CD44 is … 25/05/2017 ATCC is the complete solution supplier for toxicology From basic research through discovery and development to product testing −Continuous cell lines −Primary cells −hTERT-immortalized primary cells −upcyte ® hepatocytes (ATCC ® ACS-9000 ™) Portfolio features −Reliability −Fully characterized cells −Optimized growth protocols −Scalabilty into all aspects of the toxicology workflow −Biological … The prostate cancer cell lines LNCaP (ATCC, Manassas, VA, USA; ATCC CRL-1740) and PC3 (ATCC CRL-1435) as well as the myeloid cell lines THP-1 (ATCC TIB-202) and HL-60 (ATCC CCL-240) wereusedinthisstudy. HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. To ATCC Valued Customers, HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent.
11.10.2020
hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells(CCL-240; ATCC, Manassas, VA) were maintained at 37°C in Iscove modifiedDulbecco medium supplemented with 10% (vol/vol) FBS and 1% (vol/vol) penicillin-streptomycin in a humidified atmosphere of 95% air and 5% CO2 Differentiation of HL-60 cells into PMN-like cells was induced by incubating them in 1.25% DMSO for 6 days. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology.
model – HL-60 Study example Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type. A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for
Unopsonized and opsonized A. actinomycetemcomitans and W. recta had equally potent lethal effects on induced HL-60 cells. … I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS (non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a density if 4*105 cells/ml. Eos-HL-60 .
HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. Comments Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at 190 nM (see ATCC …
HL-60 has recently been reported to contain a sequence similar to that of the human endogenous retrovirus H/F-expressing cell line Reh (ATCC CRL-8286) (70), although there is currently no evidence that human endogenous retroviruses are expressed. ATCC HL-60, Promyelocytic Leukemia, Frozen Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. HL-60 from ATCC Description HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. Jan 17, 1989 · Although intact ATCC HL-60 cells specifically and saturably take up 1,25-dihydroxy[3H]vitamin D-3, whole cell extracts have little or no specific binding of 1,25-dihydroxyvitamin D-3. The absence of specific binding can now be explained by the action of a serine proteinase in these cells.
The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research.
Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA).
Spin down before use. Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service … hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research.
Oct 01, 2012 · HL-60 cells were obtained from the American Type Culture Collection (ATCC) and cultured in IMDM medium with 10% fetal bovine serum (FBS) at 37°C in a fully humidified atmosphere with 5%CO 2. Half of fresh medium were replaced every other day. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI Apr 15, 2010 · HL-60 cells (CCL-240) and U-937 cells (CRL-1593.2) were supplied by American Type Culture Collection (ATCC). HL-60 cultures were maintained at concentrations between 1×10 5 and 1×10 6 of viable cells/ml at 37°C under 5% CO 2 in air and 95% humidity, in Iscove's modified Dulbecco's medium (IMDM) supplemented with 20% heat-inactivated fetal Transfection of HL-60 Cells Use this procedure to transfect plasmid DNA into HL-60 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All amounts and volumes are given on a per well basis.
34 MOLT-4. 30,000.
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The human promyelocytic cell line HL-60 (ATCC CCL-240) was maintained in IMDM supplemented with 20% heat-inactivated FBS (both from Invitrogen).
ZERO BIAS - scores, article reviews, protocol conditions and more Lipofectamine® LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. This reference provides a recommended procedure to transfect plasmid DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine® LTX Reagent (Cat. No. 15338-100). 7klvsurgxfwlvlqwhqghgiruoderudwru\uhvhdufksxusrvhvrqo\ ,wlvqrwlqwhqghgiruxvhlqkxpdqv :kloh$7&&xvhvuhdvrqdeohhiiruwvwrlqfoxghdffxudwhdqgxs wr Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p Organism Type: Human: This is a non-adherent human leukocyte cell line. ATCC # CCL-240: Tissues: Lymphocyte: Phenotype: Suspension: Primary: no: Application: Cell Two types of HL-60 cells, 1,25-(OH)2D3-responsive ATCC HL-60 cells and 1,25-(OH)2D3-resistant LG HL-60 cells were used.